![]() Sera from dogs indicated to have chronic systemic disease were not included in the data set. No sex was indicated for 1% of the submissions. Samples came from intact and spayed females (33% and 21%, respectively) and intact and neutered males (23% and 21%, respectively). Median age was 8.65 years (range, 0.2 to 17.1 years). The remaining 326 pet submissions did not specify vaccine manufacturer. A subset of 105 pet dogs were known to have received the Merial/Boehringer-Ingelheim combination CAV/CPV/CDV vaccine. For the purposes of this study, Labradoodles and Goldendoodles were classified as mixed breed. Golden Retriever and Labrador Retriever samples made up 12% and 10%, respectively. Mixed-breed dogs made up 22% of the samples. Mixed-breed dogs and 73 unique pure breeds were included. The sample population included 431 pet dogs from a range of ages and breeds with known history of vaccination against canine adenovirus, canine parvovirus, and canine distemper virus as provided on the submission form. The IACUC of the University of Wisconsin School of Veterinary Medicine determined that IACUC oversight was not required for this retrospective study. Sample submission and request for testing constituted informed owner consent. Samples were submitted from across the US and Canada for quantitative testing by the Companion Animal Vaccines and ImmunoDiagnostic Service (CAVIDS) Laboratory, a fee-for-service titer-testing laboratory in the University of Wisconsin-Madison School of Veterinary Medicine. The objective of this study was to determine the diagnostic accuracy of the point-of-care dot blot ELISA (the index test) in comparison to reference standard laboratory-based assays to help guide the veterinary clinician in decisions regarding test choice. These include laboratory-based tests, which provide quantitative end point titers, as well as rapid, point-of-care, antibody-screening tests. Indications for core vaccinal antibody testing include adult dogs that are due for “booster” vaccination, any dog that has experienced an adverse reaction to vaccination in the past or other health issue, dogs that have an unknown vaccination history, puppies at least 2 weeks after the completion of the initial vaccination series, dogs in shelter outbreak situations, 4 and nomograph analysis for the breeding bitch.Īn array of antibody testing options is available to the veterinary clinician. The World Small Animal Veterinary Association also includes antibody testing as part of their vaccine guidelines. 1– American Animal Hospital Association canine vaccine guidelines 4 includes a section regarding the use of antibody testing. Antibody testing as a method to determine the potential benefit of administration of core canine viral vaccines is rapidly becoming a standard practice in veterinary medicine.
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